RNA Metabolism in Lymphocytes Stimulated by Phytohemagglutinin: Initial Responses to Phytohemagglutinin
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چکیده
منابع مشابه
Adenosine metabolism in phytohemagglutinin-stimulated human lymphocytes.
The association of a human genetic deficiency of adenosine deaminase activity with combined immunodeficiency prompted a study of the effects of adenosine and of inhibition of adenosine deaminase activity on human lymphocyte transformation and a detailed study of adenosine metabolism throughout phytohemagglutinin-induced blastogenesis. The adenosine deaminase inhibitor, coformycin, at a concentr...
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I T HAS BEEN SHOWN that peripheral i)lood lynlphocvtes undergo trainsformation and mitosis in vitro when cultured in the presence of plwtohemagglutinin ( PHA).’4 Little information has been reported on the numl)er of lymphocytes which survive and transform during the culturing interval. It has been very difficult to enumerate lymphocytes in cultures containing PFIA I)ecause the agglutinated ery...
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I TARIOUS CLINICAL AND EXPERIMENTAL STUDIES have shown V that chioramphenicol ( CAP) affects cell division. #{176} The characteristic aplastic marrow that is seen when toxicity is fully expressed suggests a toxic effect leading to depletion of dividing cells. The effect of CAP on incorporation of 32 into phytohemagglutinin ( PHA) stimulated lymphocytes was reported by McIntyre and Ebaugh,’ who ...
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By JEROME I. BRODY AND HENRY D. SOLTYS T HE PUBPOSE of this study was to delineate the durability and persistence of immunologic recall in the normal peripheral blood lymphocyte using methods which might reflect the importance of the manner by which imniunologic information is acquired. This was done in the present investigation with the technic of bacterial adherence,”2 in which circumferentia...
متن کاملEnzyme changes and polyribosome profiles in phytohemagglutinin stimulated lymphocytes.
W HEN HUMAN LYMPHOCYTES are treated with phytohemagglutinin ( PHA ) , an extract of the kidney bean Phaseolus vulgaris, they undergo morphologic transformation followed by mitosis.13 Cooper and Rubin4 have demonstrated a decrease in the amount of total RNA witin 30 minutes after the exposure of lymphocytes to PHA. This is immediately followed by an exponential increase in the synthesis of RNA w...
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ژورنال
عنوان ژورنال: Blood
سال: 1965
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v25.6.1014.1014